A01 Akira Onoda

Akira Onoda		Participating A01
Affiliation	Graduate School of Env. Science, Hokkaido University
Position	Professor
Contact	akira.onoda@ees.hokudai.ac.jp
HP	https://www.ees.hokudai.ac.jp/division/material/modules/article/biomat.html?easiestml_lang=en
Research Project	Development of Biohybrid Catalysts Having Asymmetric Coordination Sphere within a Barrel Protein
Co-Investigator	Takashi Hayashi（Professor, Graduate School of Engineering, Osaka University）
Abstract	This project aims at developing biohybrid catalysts marrying a metal complex and a protein scaffold, which provides asymmetric outer coordination sphere, to explore regio-, and chemoselective reactions. Toward this ends, we have constructed biohybrid catalysts harboring a Cp*Rh complex covalently-linked within a hydrophobic cavity of a rigid b-barrel protein, nitrobindin, and will explore to perform C−C bond-forming reactions including isoquinoline synthesis using the Rh-linked biohybrid catalysts. We have also constructed a unique p-expanded reaction cavity tethering a polycyclic moiety which provides a platform for substrate binding within the robust b-barrel structure of nitrobindin. The nitrobindin variants with the cavities of different sizes and shapes are coupled with a pyrene moiety to prepare a series of biohybrid catalysts. The Diels-Alder reaction between azacalcone and cyclopentadiene and other reactions will be tested within the pyrene-linked reaction cavity.
Research Areas	Coordination Chemistry, Bioinorganic Chemistry, Hybrid Materials
Keywords	Biohybrid Catalyst, Asymmetric Coordination Sphere, Proteins

2020

[8] Triazolecarbaldehyde Reagents for One-step N-Terminal Protein Modification,
Akira Onoda; Nozomu Inoue; Eigo Sumiyoshi; Takashi Hayashi, ChemBioChem, 21, 1274 – 1278, 2020
DOI: 10.1002/cbic.201900692.

2019

[7] Site-specific Modification of Proteins through N-terminal Azide-labeling and a Chelation-assisted CuAAC Reaction,
Nozomu Inoue; Akira Onoda; Takashi Hayashi, Bioconjugate Chem., 30, 2427 – 2439, 2019
DOI: 10.1021/acs.bioconjchem.9b00515.

2018

[6] A Water-Soluble Supramolecular Complex that Mimics the Heme/Copper Hetero-Binuclear Site of Cytochrome c Oxidase,
Hiroaki Kitagishi; Daiki Shimoji; Takehiro Ohta; Ryo Kamiya; Yasuhiro Kudo; Akira Onoda; Takashi Hayashi; Jean Weiss; Jennifer A. Wytko; Koji Kano, Chem. Sci., 9, 1989 – 1995, 2018
DOI: 10.1039/C7SC04732K.

[5] Mitochondrial-targeting Polyamine−Protoporphyrin Conjugates for Photodynamic Therapy,
Fargol Taba; Akira Onoda; Urara Hasegawa; Toshiaki Enoki; Yousuke Ooyama; Joji Ohshita; Takashi Hayashi, ChemMedChem, 12, 15 – 18, 2018
DOI: 10.1002/cmdc.201700467.

[4] Nonprecious-metal Fe/N/C Catalysts Prepared from p-Expanded Fe Salen Precursors toward an Efficient Oxygen Reduction Reaction,
Yuta Tanaka; Akira Onoda; Shin-ichi Okuoka; Tomoaki Kitano; Koki Matsumoto; Takao Sakata; Hidehiro Yasuda; Takashi Hayashi, ChemCatChem, 9, 743 – 750, 2018
DOI: 10.1002/cctc.201701629.

[3] Bimetallic M/N/C Catalysts Prepared from π-Expanded Metal Salen Precursors toward an Efficient Oxygen Reduction Reaction,
Akira Onoda; Yuta Tanaka; Koki Matsumoto; Minoru Ito; Takao Sakata; Hidehiro Yasuda; Takashi Hayashi, RSC Adv., 8, 2892 – 2899, 2018
DOI: 10.1039/c7ra12657c.

2017

[2] Cofactor-specific Anchoring of Horseradish Peroxidase onto a Polythiophene-modified Electrode,
Akira Onoda; Yasunari Umeda; Takashi Hayashi, Chem. Lett., 46, 1807 – 1809, 2017
DOI: 10.1246/cl.170837.

[1] A Pyrene-linked Cavity within a beta-Barrel Protein Promotes an Asymmetric Diels-Alder Reaction,
Tomoki Himiyama; Naomasa Taniguchi; Shunsuke Kato; Akira Onoda; Takashi Hayashi, Angew. Chem. Int. Ed., 56, 13618 – 13622, 2017
DOI: 10.1002/anie.201704524.